Journal: Gut microbes
Article Title: Helminth reshapes host gut microbiota and immunoregulation by deploying an antimicrobial program of innate immunity.
doi: 10.1080/19490976.2025.2496447
Figure Lengend Snippet: Figure 2. CRAMP drives the expansion of lactobacilli bacteria during Emu infection. (a) ELISA measurements of antibacterial peptides (AMPs) in serum and colon tissue in the naive mice and mice infected with Emu 6 weeks post-infection. The expression levels were scaled between naive and infection and are shown in Z-score. (b) The protein level of CRAMP in the serum of the mice at the early or chronic infection stage. (c) The increased level of CRAMP in the feces of the mice 2 hours after injection with mCRAMP. (d and e) the abundances of Lactobacillus and Enterobacteriaceae (d) and L/E ratio (e) in the feces of the mice injected with mouse CRAMP or vehicle control. qPCR analyses were performed every two days. (f, g, and h) the abundances of Lactobacillus (f) and Enterobacteriaceae (g) and
Article Snippet: The level of each antibacterial peptide was evaluated using ELISA methods as per the recommendation of each manufacturer, including CRAMP ELISA kit (Cat. CSB-E5061m, Cusabio), Reg3g ELISA kit (Cat. CSB-EL9549MO, Cusabio), Sprr2a ELISA kit (Cat. K8–14835, KIRbio), Lyz1 ELISA kit (Cat. K8–14839), Retnla ELISA kit (Cat. K8–02975), α-defensins ELISA kit (Cat. K6–02404, KIRbio), β-defensins ELISA kit (Cat. K6–02440, KIRbio), Reg3α ELISA kit (Cat. K6–14918, KIRbio), and human LL-37 ELISA kit (Cat. CSBEL4476HU, Cusabio).
Techniques: Bacteria, Infection, Enzyme-linked Immunosorbent Assay, Expressing, Injection, Control